Typical dynamic binding capacity
IMAC
Test: Papain capacity vs linear velocity
Feed solution: 20 g/l Papain crude extract in 20 mM phosphate buffer, pH 7,2 + NaCl 200 mM
Buffer equilibration: 12 BV phosphate buffer, pH 7,2 + NaCl 200 mM
Dispacement: 8 BV phosphate buffer, pH 7,2 + imidazole NaCl 0.5 M
Elution: 4 BV phosphate buffer, pH 7,2 + NaCl 200 mM
IDA400/SS
- Introduction
- Technical information
- Instructions for use
- Remarks Typical dynamic binding capacity
- AEX
- HIC
- CEX
- IMAC
- Purchase Information
- Material Support File
- HICPR: typical examples
