Typical dynamic binding capacity
HIC
Test: BSA capacity vs linear velocity
Feed solution: 10 g/l BSA in 20 mM phosphate buffer pH 7 + (NH4)2SO4 2M
Buffer equilibration: 6 BV of 20 mM phosphate buffer pH 7 + (NH4)2SO4 2M
BSA loading: 10 BV
Dispacement: 6 BV of 20 mM phosphate buffer pH 7 + (NH4)2SO4 2M
Elution: 4 BV of 20mM phosphate buffer pH 7
BU400/SS
PH400/SS
OD400/SS
- Introduction
- Technical information
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- Remarks Typical dynamic binding capacity
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- HICPR: typical examples