Typical dynamic binding capacity


AEX


Test: BSA capacity vs linear velocity

Feed solution: 10 g/l BSA in 20 mM TRIS-HCl buffer pH = 7
Buffer equilibration: 6 BV of 20 mM TRIS HCl buffer pH = 7
BSA loading: 12 BV
Dispacement: 6 BV of 20 mM TRIS HCl buffer pH = 7
Elution: 6 BV of TRIS-HCl buffer pH = 7 + 4 BV of NaCl 2M

QA400/SS


DA400/SS