ReliChrom™ Documentation
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ReliChrom™
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ReliChrom™ Documentation
Russian version
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ReliChrom™
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IMAC
Test: Papain capacity vs linear velocity
IMAC
| Feed solution: | 20 g/l Papain crude extract in 20 mM phosphate buffer, pH 7,2 + NaCl 200 mM |
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| Buffer equilibration: | 12 BV phosphate buffer, pH 7,2 + NaCl 200 mM | |
| Dispacement: | 8 BV phosphate buffer, pH 7,2 + imidazole NaCl 0.5 M | |
| Elution: | 4 BV phosphate buffer, pH 7,2 + NaCl 200 mM |
IDA400/SS
