IMAC
Test: Papain capacity vs linear velocity
IMAC
Feed solution: | 20 g/l Papain crude extract in 20 mM phosphate buffer, pH 7,2 + NaCl 200 mM |
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Buffer equilibration: | 12 BV phosphate buffer, pH 7,2 + NaCl 200 mM | |
Dispacement: | 8 BV phosphate buffer, pH 7,2 + imidazole NaCl 0.5 M | |
Elution: | 4 BV phosphate buffer, pH 7,2 + NaCl 200 mM |
IDA400/SS
ReliChrom™
- Introduction
- Technical information
- Instructions for use
- Remarks
- Typical dynamic binding capacity (DBC)
- Purchase information
- Material Support File
- HICPR: typical examples