Resindion Shop Login

ReliChrom™ Documentation

image

ReliChrom™
Products line brochure

English version PDF

ReliChrom™ Documentation
Russian version

image

ReliChrom™
Products line brochure

Russian version PDF

Instructions for use

ReliChrom

The flow diagram (fig. 1) shows schematically all the steps to perform a standard capture or polishing procedure on ReliChrom™ columns.

Preliminary set up:

  • Rinse the chromatographic system circuit with DI water;
  • After the removal of the upper stopper of the ReliChrom™ column, connect it to the chromatographic unit;
  • Remove the bottom stopper of ReliChrom™ column and connect the column outlet to the specific device of the chromatographic system (Detector, fraction collector…).

 

Resindio srl - ReliChrom™ scheme istruction for us

Wash out the conditioning solution with 10 BV OF Dl waater;

 

Start the equilibration with the desired buffer solution at an appropriate linear flow rate;

 

Run the chromatographic separation, according to your individual protocol at the same flow rate in step 2;

 

If necessary, perform a regeneration according to the table below.

 

Resin type Standard Regeneration steps
AEX

• Regenerate with 1 ÷ 1.5 BV of NaOH 0.5 ÷ 1 M
• Displace the regenerant with 2 BV of DI water
• Only for strong AEX, condition the resin with 2 BV NaCl 0.5 ÷ 1M
• Rinse with 5 – 10 BV of DI water

IDA • Remove residual Ni with EDTA 50 mM
• Displace EDTA with 2 BV of DI water
• Condition the resin with 1 - 1.5 BV of NaOH 0.5 M
• Wash the resin with 5 – 10 BV of DI water
• Reload Ni with a suitable salt solution (NiCl2)
CEX

• Condition the resin with 1 BV of NaOH 0.5 M
• Displace the base with 2 BV of DI water
• Recondition with 1 – 1.5 BV HCl 0.5 M
• Displace the acid with 2 BV of DI water
• Only for strong CEX, condition the resin with 2 BV NaCl 0.5 - 1 M
• Rinse with 5 - 10 BV ol BI water

HIC

• Wash the resin with 2 BV of DI water
• Clear the resin with 1 BV of NaOH 0.5 M in DI water or in 10+40% alcohol solution
• Displace NaOH solution with 2 BV of DI water
• Rinse with 5 BV ol BI water

 

ReliChrom

IMPORTANT

Sales through Resindion shop are made in accordance with the "On-Line Terms reported in the terms and conditions of sales".
Buyer before proceeding with any purchase must agree to the rules.

On this site we use cookies to enhance your experience when using it. To learn more about the cookies we use, view our privacy policy.

I accept cookies from this site.