Click to enlarge

¹Ni²⁺ ionic form is applied only for ReliChrom™ pre-packed columns; different ionic forms are also available upon request.
^aFeed solution: 10 g/l BSA in 20 mM TRIS - HCl buffer, pH 7; flow rate = 150 cm/h.
^bFeed solution: 10 g/l BSA in 20 mM phosphate buffer,
pH 7 + (NH₄)₂SO₄ 2 M; flow rate = 150 cm/h.
^cFeed solution: 8 g/l Lysozyme in 20 mM sodium acetate buffer, pH 5; flow rate = 150 cm/h.
^dFeed solution: 20 g/l Papaine crude extract in 20 mM phosphate buffer, pH 7.2 + NaCl 200 mM; flow rate = 150 cm/h.

Supply conditions

• IEX and IMAC columns: EtOH 20% aqueous solution + NaCl (final concentration 150 mM)
• HIC columns: EtOH 20% aqueous solution