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ReliSorb™ Documentation

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ReliSorb™
Products line brochure

English version PDF

ReliSorb™ Documentation Russian version

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ReliSorb™
Products line brochure

Russian version PDF

Ion Exchange resins

Resindion srl - ReliSorb™ product line

Ion exchange chromatography is the most common method applied for the separation and the purification of biomolecules.
The electrostatic interaction between a charged solute and a counter charge covalently bounded onto the resin is the base of this method of purification.
The use of a weak ion exchanger is determined by the ionization degree at the pH value at which the bio-molecule has a net charge. On the contrary, strong ion exchangers, being completely ionized, can be used over a wide range of pH.
In theory a weak cation exchanger should be used above pH 5.0-5.5, while a weak anion one at pH below 8.0-8.5.
A raw solution with low conducibility is preferably loaded on a buffered resin, while a low molarity buffer with salt gradient or pH change is used for the elution.

Strong cation
Exchanger
Weak cation
Exchanger
Funcional group
-SO3Na
Funcional group
-COOH
Resindion srl - Relisorb™ strong cation exchanger Resindion srl - reliSorb™ Weak cation Exchanger

 

Strong anion
Exchanger
Weak anion
Exchanger
Funcional group
-N+R3Cl-, R = CH3
Funcional group
-NR2, R = CH2CH3
Resindion srl ReliSorb™ Strong anion Exchanger Resindion srl - Relisorb™ Weak anion Exchanger

 

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